Volume 4, Issue 4 (2018)
IEM 2018, 4(4): 153-157 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Bahroudi S, Nematollahi M, Aghasadeghi M, Nazemi M. In vitro Evaluation of the Antiviral Activity and Cytotoxicity Effect of Holothuria leucospilota Sea Cucumber Extracts from the Persian Gulf. IEM 2018; 4 (4) :153-157
URL: http://iem.modares.ac.ir/article-4-26525-en.html
URL: http://iem.modares.ac.ir/article-4-26525-en.html
1- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran
2- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran ,mallahi@ut.ac.ir
3- Hepatitis & AIDS Departments, Pasteur Institute of Iran, Tehran, Iran
4- Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
2- Fisheries Department, Natural Resources Faculty, University of Tehran, Karaj, Iran ,
3- Hepatitis & AIDS Departments, Pasteur Institute of Iran, Tehran, Iran
4- Persian Gulf & Oman Sea Ecological Research Institute, Bandar Abbas, Hormozgan, Iran
Abstract: (3355 Views)
Aims: Antiviral activity and effect of methanol and diethyl ether extracts from different parts of sea cucumber (Holothuria leucospilota) against HIV-1 were assessed on human oral epidermoid carcinoma cells (KB) and Human embryonic kidney 293T cells (HEK293T).
Materials & Methods: Sea cucumber was collected at a depth of 10-30 m (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of single-cycle HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed, using a cell proliferation XTT kit.
Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less effect.
Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall and antiviral activity, respectively.
Materials & Methods: Sea cucumber was collected at a depth of 10-30 m (Persian Gulf). Extracts were prepared by diethyl ether and methanol solvents. The antiviral activity of each extract was evaluated by inhibition of single-cycle HIV-1 (SCR HIV-1) p24 Core antigen production in HeLa cells and cellular toxicity of different extracts were assessed, using a cell proliferation XTT kit.
Findings: Antiviral activity of each extract showed that some concentrations were able to inhibit the replication of HIV-1. Diethyl ether extract of body wall with 2.79 TI index displayed the highest antiviral activity as well as less effect.
Conclusion: This study showed that crude extracts of Holothuria leucospilota, especially methanol and diethyl ether extracts of digestive organs and body wall and antiviral activity, respectively.
Article Type: Original Research |
Subject:
Virology
Received: 2018/05/27 | Accepted: 2018/10/8 | Published: 2018/12/20
Received: 2018/05/27 | Accepted: 2018/10/8 | Published: 2018/12/20
References
1. Faulkner DJ. Marine natural products. Nat Prod Rep. 1996;13(2):75-125. [Link] [DOI:10.1039/np9961300075]
2. Bordbar S, Anwar F, Saari N. High-value components and bioactives from sea cucumbers for functional foods - a review. Mar Drugs. 2011;9(10):1761-805. [Link] [DOI:10.3390/md9101761]
3. Bryan PJ, McClintock JB, Hopkins TS. Structural and chemical defenses of echinoderms from the northern Gulf of Mexico. J Exp Mar Biol Ecol. 1997;210(2):173-86. [Link] [DOI:10.1016/S0022-0981(96)02677-9]
4. Villasin J, Pomory CM. Antibacterial activity of extracts from the body wall of Parastichopus parvimensis (Echinodermata: Holothuroidea). Fish Shellfish Immunol. 2000;10(5):465-7. [Link] [DOI:10.1006/fsim.2000.0265]
5. Farouk AEA, Hamid Ghouse FA, Ridzwan BH. New bacterial species isolated from Malaysian sea cucumbers with optimized secreted antibacterial activity. Am J Biochem Biotechnol. 2007;3(2):60-5. [Link] [DOI:10.3844/ajbbsp.2007.60.65]
6. Tian F, Zhang X, Tong Y, Yi Y, Zhang S, Li L, et al. PE, a new sulfated saponin from sea cucumber, exhibits anti-angiogenic and anti-tumor activities in vitro and in vivo. Cancer Biol Ther. 2005;4(8):874-82. [Link] [DOI:10.4161/cbt.4.8.1917]
7. Maier MS, Roccatagliata AJ, Kuriss A, Chludil H, Seldes AM, Pujol CA, et al. Two new cytotoxic and virucidal trisulfated triterpene glycosides from the Antarctic sea cucumber Staurocucumis liouvillei. J Nat Prod. 2001;64(6):732-6. [Link] [DOI:10.1021/np000584i]
8. UNAIDS. Global report: UNAIDS report on the global AIDS epidemic 2010 [Internet]. Geneva: UNAIDS; 2010 [cited 2015 Jul 2]. Available from: http://www.unaids.org/globalreport/ [Link]
9. Naik CG, Kamat SY, Parameswaran PS, Das B, Bhattacharya J, Ramani P, et al. Bioactivity of marine organisms: IV. Screening of some marine animals from the Indian coast. Mahasagar. 1989;22(2):99-104. [Link]
10. Zabihollahi R, Sadat SM, Vahabpour R, Aghasadeghi MR, Memarnejadian A, Ghazanfari T, et al. Development of single-cycle replicable human immunodeficiency virus 1 mutants. Acta Virol. 2011;55(1):15-22. [Link] [DOI:10.4149/av_2011_01_15]
11. Cheng PW, Ng LT, Chiang LC, Lin CC. Antiviral effects of saikosaponins on human coronavirus 229E in vitro. Clin Exp Pharmacol Physiol. 2006;33(7):612-6. [Link] [DOI:10.1111/j.1440-1681.2006.04415.x]
12. Huang N, Wu MY, Zheng CB, Zhu L, Zhao JH, Zheng YT. The depolymerized fucosylated chondroitin sulfate from sea cucumber potently inhibits HIV replication via interfering with virus entry. Carbohydr Res. 2013;380:64-9. [Link] [DOI:10.1016/j.carres.2013.07.010]
13. Farshidpour F, Gharibi S, Taherzadeh M, Amirinejad R, Taherkhani R, Habibian A, et al. Antiviral activity of Holothuria sp. a sea cucumber against herpes simplex virus type 1 (HSV-1). Eur Rev Med Pharmacol Sci. 2014;18(3):333-7. [Link]
14. Sugawara T, Zaima N, Yamamoto A, Sakai S, Noguchi R, Hirata T. Isolation of sphingoid bases of sea cucumber cerebrosides and their cytotoxicity against human colon cancer cells. Biosci Biotechnol Biochem. 2006;70(12):2906-12. [Link] [DOI:10.1271/bbb.60318]
15. Rodriguez J, Castro R, Riguera R. Holothurinosides: New antitumour non sulphated triterpenoid glycosides from the sea cucumber Holothuria forskalii. Tetrahedron. 1991;47(26):4753-62. [Link] [DOI:10.1016/S0040-4020(01)86479-6]
16. Chludil HD, Murray AP, Seldes AM, Maier MS. Biologically active triterpene glycosides from sea cucumbers (Holothuroidea, Echinodermata). In: Rahman Au, editor. Studies in natural products chemistry: Bioactive natural products, part 1. 28th Volume. Amsterdam: Elsevier Science; 2003. pp. 587-615. [Link]
17. Han H, Li L, Yi Y, Wang X, Pan M. Triterpene glycosides from sea cucumber Holothuria scabra with cytotoxic activity. Chin Herb Med. 2012;4(3):183-8. [Link]
18. Althunibat O, Ridzwan B, Taher M, Daud JM, Jauhari Arief Ichwan S, Qaralleh H. Antioxidant and cytotoxic properties of two sea cucumbers, Holothuria edulis Lesson and Stichopus horrens Selenka. Acta Biologica Hungarica 2013;64(1):10-20. [Link] [DOI:10.1556/ABiol.64.2013.1.2]
19. Yang P, Collin P, Madden T, Chan D, Sweeney-Gotsch B, McConkey D, et al. Inhibition of proliferation of PC3 cells by the branched-chain fatty acid, 12-methyltetradecanoic acid, is associated with inhibition of 5-lipoxygenase. Prostate. 2003;55(4):281-91. [Link] [DOI:10.1002/pros.10243]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |