Volume 4, Issue 4 (2018)                   IEM 2018, 4(4): 147-152 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shariat Bahadori E, Sadraei J, Dalimi A. Evaluation of NT-ProBNP and Procalcitonin as Markers from Rodents with Myocardial Toxoplasmosis Using ECL Method: A Pilot Study. IEM. 2018; 4 (4) :147-152
URL: http://iem.modares.ac.ir/article-4-27073-en.html
1- Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2- Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran , sadraeij@modares.ac.ir
Abstract:   (4030 Views)
Aims: Toxoplasma parasites that extracted from different rodents are the same in immunologic and morphological characteristics but different in pathogenic characteristics. We found that the serum levels of ProBNP and Procalcitonin markers are high among these rodents. The aim of this study was the assessment of the serum levels of ProBNP and Procalcitonin markers among the rodents with myocardial .
Materials & Methods: In this study, we collected 286 rodents and extracted 250g of their heart tissues and blood samples to obtain DNA of T. . We detected the positive samples, using the nested PCR method. Then, we examined serum levels of Pro BNP and Procalcitonin markers, using Electro Chemo Luminescence method (ECL) for assessment of myocardial in this host. Data analysis was also conducted by the statistical analysis method. This study was performed from January to March 2017, based on the prevalence study.
Findings: In this study, 68/286 samples of rodents were positive for GRA6 gene and these positive samples had high levels of Pro BNP and Procalcitonin markers that indicated myocardial and acute inflammation among these animals.
Conclusion: In this study, we found that the GRA6 gene was very useful to follow up in the rodents of the Golestan province, northeast of Iran. Also, ProBNP and Procalcitonin markers were at high levels in myocardial .
Full-Text [PDF 749 kb]   (504 Downloads)    
Article Type: Original Research | Subject: Parasitology
Received: 2018/05/11 | Accepted: 2018/10/6 | Published: 2018/12/20

1. Selseleh M, Keshavarz H, Mohebali M, Shojaee S, Modarressi MH, Eshragian MR, et al. Production and evaluation of Toxoplasma gondii recombinant surface antigen 1 (SAG1) for serodiagnosis of acute and chronic Toxoplasma infection in human sera. Iran J Parasitol. 2012;7(3):1-9. [Link]
2. Lim H, Lee SE, Jung BK, Kim MK, Lee MY, Nam HW, et al. Serologic survey of toxoplasmosis in Seoul and Jeju-do, and a brief review of its seroprevalence in Korea. Korean J Parasitol. 2012;50(4):287-93. [Link] [DOI:10.3347/kjp.2012.50.4.287]
3. Lee SE, Hong SH, Lee SH, Jeong YII, Lim SJ, Kwon OW, et al. Detection of ocular Toxoplasma gondii infection in chronic irregular recurrent uveitis by PCR. Korean J Parasitol. 2012;50(3):229-31. [Link] [DOI:10.3347/kjp.2012.50.3.229]
4. El Behairy AM, Choudhary S, Ferreira LR, Kwok OC, Hilali M, Su C, et al. Genetic characterization of viable Toxoplasma gondii isolates from stray dogs from Giza, Egypt. Vet Parasitol. 2013;193(1-3):25-9. [Link] [DOI:10.1016/j.vetpar.2012.12.007]
5. Edwards JF, Dubey JP. Toxoplasma gondii abortion storm in sheep on a Texas farm and isolation of mouse virulent atypical genotype T. gondii from an aborted lamb from a chronically infected ewe. Vet Parasitol. 2013;192(1-3):129-36. [Link] [DOI:10.1016/j.vetpar.2012.09.037]
6. Dubey JP, Choudhary S, Kwok OC, Ferreira LR, Oliveira S, Verma SK, et al. Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus olor) from the USA. Vet Parasitol. 2013;195(1-2):42-6. https://doi.org/10.1016/j.vetpar.2012.12.051 [Link] [DOI:10.1016/j.vetpar.2006.04.037]
7. Herrmann DC, Wibbelt G, Götz M, Conraths FJ, Schares G. Genetic characterisation of Toxoplasma gondii isolates from European beavers (Castor fiber) and European wildcats (Felis silvestris silvestris). Vet Parasitol. 2013;191(1-2):108-11. [Link] [DOI:10.1016/j.vetpar.2012.08.026]
8. Lilly EL, Wortham CD. High prevalence of Toxoplasma gondii oocyst shedding in stray and pet cats (Felis catus) in Virginia, United States. Parasit Vectors. 2013;6:266. [Link] [DOI:10.1186/1756-3305-6-266]
9. Zhang XX, Lou ZZ, Huang SY, Zhou DH, Jia WZ, Su C, et al. Genetic characterization of Toxoplasma gondii from Qinghai vole, Plateau pika and Tibetan ground-tit on the Qinghai-Tibet Plateau, China. Parasit Vectors. 2013;6:291. [Link] [DOI:10.1186/1756-3305-6-291]
10. Cabral AD, Gama AR, Sodré MM, Savani ES, Galvão-Dias MA, Jordão LR, et al. First isolation and genotyping of Toxoplasma gondii from bats (Mammalia: Chiroptera). Vet Parasitol. 2013;193(1-3):100-4 [Link] [DOI:10.1016/j.vetpar.2012.11.015]
11. Mancianti F, Nardoni S, D'Ascenzi C, Pedonese F, Mugnaini L, Franco F, et al. Seroprevalence, detection of DNA in blood and milk, and genotyping of Toxoplasma gondii in a goat population in Italy. Biomed Res Int. 2013;2013:905326. [Link]
12. Ortega-Pacheco A, Acosta Viana KY, Guzmán-Marín E, Segura-Correa JC, Alvarez-Fleites M, Jiménez-Coello M. Prevalence and risk factors of Toxoplasma gondii in fattening pigs farm from Yucatan, Mexico. Biomed Res Int. 2013;2013:231497. [Link]
13. Galván-Ramírez Mde L, Sánchez-Orozco LV, Rodríguez LR, Rodríguez S, Roig-Melo E, Troyo Sanromán R, et al. Seroepidemiology of Toxoplasma gondii infection in drivers involved in road traffic accidents in the metropolitan area of Guadalajara, Jalisco, Mexico. Parasit Vectors. 2013;6(1):294. [Link] [DOI:10.1186/1756-3305-6-294]
14. Xu S, Zhang C, He L, Wang T, Ni L, Sun M, et al. DNA detection of Toxoplasma gondii with a magnetic molecular beacon probe via CdTe@Ni quantum dots as energy donor. J Nanomater. 2013;2013:473703. [Link]
15. Cong W, Huang SY, Zhou DH, Zhang XX, Zhang NZ, Zhao Q, et al. Prevalence and genetic characterization of Toxoplasma gondii in house sparrows (Passer domesticus) in Lanzhou, China. Korean J Parasitol. 2013;51(3):363-7. [Link] [DOI:10.3347/kjp.2013.51.3.363]
16. Khademvatan S, Saki J, Yousefi E, Abdizadeh R. Detection and genotyping of Toxoplasma gondii strains isolated from birds in the Southwest of Iran. Br Poult Sci. 2013;54(1):76-80. [Link] [DOI:10.1080/00071668.2013.763899]
17. Jenks SJ, Conway BR, Mc Lachlan S, Teoh WL, Williamson RM, Webb DJ, et al. Cardiovascular disease biomarkers are associated with declining renal function in type 2 diabetes. Diabetologia. 2017;60(8):1400-8. [Link] [DOI:10.1007/s00125-017-4297-0]
18. Chokengarmwong N, Yeh DD, Chang Y, Ortiz LA, Kaafarani HMA, Fagenholz P, et al. Elevated admission N-terminal pro-brain natriuretic peptide level predicts the development of atrial fibrillation in general surgical intensive care unit patients. J Trauma Acute Care Surg. 2017;83(3):485-90. [Link] [DOI:10.1097/TA.0000000000001552]
19. Taylor CJ, Monahan M, Roalfe AK, Barton P, Iles R, Hobbs FDR. The REFER (REFer for EchocaRdiogram) study: A prospective validation and health economic analysis of a clinical decision rule, NT-proBNP or their combination in the diagnosis of heart failure in primary care. Effic Mech Eval. 2017 Apr. 28- Uçkay I, Garzoni C, Ferry T, Harbarth S, Stern R, Assal M, et al. Postoperative serum pro-calcitonin and C-reactive protein levels in patients with orthopedic infections. Swiss Med Wkly. 2010;140:w13124. [Link]
20. Lewis LK, Raudsepp SD, Yandle TG, Prickett TC, Richards AM. Development of a BNP1-32 immunoassay that does not cross-react with proBNP. Clin Chem. 2017;63(6):1110-7. [Link] [DOI:10.1373/clinchem.2016.269712]
21. Tu WJ, Ma GZ, Ni Y, Hu XS, Luo DZ, Zeng XW, et al. Copeptin and NT-proBNP for prediction of all-cause and cardiovascular death in ischemic stroke. Neurology. 2017;88(20):1899-905. [Link] [DOI:10.1212/WNL.0000000000003937]
22. Popelová JR, Tomková M, Tomek J. NT-proBNP predicts mortality in adults with transposition of the great arteries late after Mustard or Senning correction. Congenit Heart Dis. 2017;12(4):448-57. [Link] [DOI:10.1111/chd.12466]
23. Jia J, Chang GL, Qin S, Chen J, He WY, Lu K, et al. Comparative evaluation of copeptin and NT-proBNP in patients with severe acute decompensated heart failure, and prediction of adverse events in a 90-day follow-up period: A prospective clinical observation trial. Exp Ther Med. 2017;13(4):1554-60. [Link] [DOI:10.3892/etm.2017.4111]
24. Potter JM, Simpson AJ, Kerrigan J, Southcott E, Salib MM, Koerbin G, et al. The relationship of plasma creatinine (as eGFR) and high-sensitivity cardiac troponin and NT-proBNP concentrations in a hospital and community outpatient population. Clin Biochem. 2017;50(15):813-5. [Link] [DOI:10.1016/j.clinbiochem.2017.04.003]
25. Hwang JW, Park SJ, Cho EJ, Kim EK, Lee GY, Chang SA, et al. Relation of N-Terminal Pro-B-Type natriuretic peptide and left ventricular diastolic function to exercise tolerance in patients with significant valvular heart disease and normal left ventricular systolic function. Am J Cardiol. 2017;119(11):1846-53. [Link] [DOI:10.1016/j.amjcard.2017.02.038]
26. Bijsmans ES, Jepson RE, Wheeler C, Syme HM, Elliott J. Plasma N-Terminal probrain natriuretic peptide, vascular endothelial growth factor, and cardiac troponin i as novel biomarkers of hypertensive disease and target organ damage in cats. J Vet Intern Med. 2017;31(3):650-60. [Link] [DOI:10.1111/jvim.14655]
27. Akcay I, Okoh AK, Yalav O, Eray IC, Rencuzogullari A, Dalci K, et al. The prognostic value of pro-calcitonin, CRP and thyroid hormones in secondary peritonitis: a single-center prospective study. Ulus Travma Acil Cerrahi Derg. 2014;20(5):343-52. [Link] [DOI:10.5505/tjtes.2014.98354]
28. Uçkay I, Garzoni C, Ferry T, Harbarth S, Stern R, Assal M, et al. Postoperative serum pro-calcitonin and C-reactive protein levels in patients with orthopedic infections. Swiss Med Wkly. 2010;140:w13124. [Link] [DOI:10.4414/smw.2010.13124]
29. Mol JA, Kwant MM, Arnold IC, Hazewinkel HA. Elucidation of the sequence of canine (pro)-calcitonin, a molecular biological and protein chemical approach. Regul Pept. 1991;35(3):189-95. [Link] [DOI:10.1016/0167-0115(91)90082-R]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.