Volume 9, Issue 2 (2023)
IEM 2023, 9(2): 179-190 |
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Adekola H, Ojo D, Balogun S, Dipeolu M, Mohammed M, Amusan A. Seroprevalence of Zika Virus IgM Antibodies in Pregnant Woman in Nigeria. IEM 2023; 9 (2) :179-190
URL: http://iem.modares.ac.ir/article-4-67183-en.html
URL: http://iem.modares.ac.ir/article-4-67183-en.html
Hafeez Adekola * 
1, David Ojo2 , Saka Balogun2 , Morenike Dipeolu3 , Musa Mohammed4 , Adeolu Amusan5
1, David Ojo2 , Saka Balogun2 , Morenike Dipeolu3 , Musa Mohammed4 , Adeolu Amusan5
1- Department of Microbiology, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria , adekola.hafeez@oouagoiwoye.edu.ng
2- Department of Microbiology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
3- Department of Veterinary Public Health and Reproduction, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
4- Department of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
5- Veterinary Teaching Hospital, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
2- Department of Microbiology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
3- Department of Veterinary Public Health and Reproduction, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
4- Department of Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
5- Veterinary Teaching Hospital, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria
Abstract: (598 Views)
Backgrounds: In developing countries like Nigeria, screening of Zika virus (ZIKV) infection in pregnant women remains limited due to a lack of diagnostic facilities and non-specific symptoms, leading to potential misdiagnosis of the disease as other febrile illnesses such as malaria or typhoid.
Materials & Methods: To address this issue, this study aimed to investigate the prevalence of anti-ZIKV IgM antibodies in pregnant women using enzyme-linked immunoassay. Additionally, the quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay targeted a specific region of the membrane protein (prM) gene to detect Zika virus presence in the collected serum samples. For a period of four months from December 2021 to March 2022, a total of 360 serum samples were collected from pregnant women attending antenatal care units in two tertiary hospitals located in different regions of Nigeria.
Findings: The results of this study revealed a prevalence of 17.2% (62 samples) for anti-ZIKV IgM antibodies among pregnant women. Further analysis using the RT-qPCR method detected Zika virus (prM gene) in 1.9% (7/62) of the serum samples. In addition to these virological results, the statistical analysis of sociodemographic data, clinical characteristics, and risk factors for ZIKV infection demonstrated a significant correlation between seropositivity and various factors including ethnicity, residence, occupation, and history of arboviral diseases (p< .005).
Conclusion: Given the potential consequences of ZIKV infection in pregnant women, early diagnosis and intervention could improve maternal outcomes and prevent fetal abnormalities.
Materials & Methods: To address this issue, this study aimed to investigate the prevalence of anti-ZIKV IgM antibodies in pregnant women using enzyme-linked immunoassay. Additionally, the quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay targeted a specific region of the membrane protein (prM) gene to detect Zika virus presence in the collected serum samples. For a period of four months from December 2021 to March 2022, a total of 360 serum samples were collected from pregnant women attending antenatal care units in two tertiary hospitals located in different regions of Nigeria.
Findings: The results of this study revealed a prevalence of 17.2% (62 samples) for anti-ZIKV IgM antibodies among pregnant women. Further analysis using the RT-qPCR method detected Zika virus (prM gene) in 1.9% (7/62) of the serum samples. In addition to these virological results, the statistical analysis of sociodemographic data, clinical characteristics, and risk factors for ZIKV infection demonstrated a significant correlation between seropositivity and various factors including ethnicity, residence, occupation, and history of arboviral diseases (p< .005).
Conclusion: Given the potential consequences of ZIKV infection in pregnant women, early diagnosis and intervention could improve maternal outcomes and prevent fetal abnormalities.
Article Type: Original Research |
Subject:
Virology
Received: 2023/01/31 | Accepted: 2023/05/26 | Published: 2023/08/19
Received: 2023/01/31 | Accepted: 2023/05/26 | Published: 2023/08/19
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